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KMID : 0380620190510060517
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 6 p.517 ~ p.523
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Antioxidant activities and Validation of Analytical Method of Marker compounds in strawberry fruits from various cultivars
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Yong Ye-Seul
Lee Song-Mi
Byun Na-Young
Sun Sang-Ouk
Kim Min-Jung
Jang Seo-Woo
Jang Won-Suk
Lee Sun-Yi
Yim Soon-Ho
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Abstract
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In this study, we investigated antioxidant capacity of 20 strawberry extract using the DPPH and ABTS assay and HPLC-DAD validation method. The total polyphenolic and flavonoids contents of 20 strawberry extracts were 22.77- 107.61 mg TAE/100 g FW and 17.58-44.12 mg QE/100 g FW. The Aiberry and Elie star showed the highest total polyphenol and flavonoid contents, respectively. The DPPH radical scavenging activity of what was 1540.6-1124.0 μmol TEAC/100 g FW and Derunoka showed the highest activity. The ABTS radical scavenging activity was 6352.3-4592.3 μmol TEAC/100 g FW and FA23 showed the highest activity. The HPLC-DAD method for the quantitation of ellagic acid results showed high linearity in various concentration ranges, and the limit of detection was 2.35 μg/mL. The limit of quantification was 7.12 μg/ mL. Relative standard deviation values from intra-and inter-day precision were less than 5.31%. Recovery rate at 10, 50, and 100 μg/mL, respectively, were 100.0-101.0% with relative standard deviation (RSD) values less than 5.30%. These results provide viable information for the validation of antioxidant capacity in strawberry fruits.
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KEYWORD
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strawberry, ellagic acid, antioxidant, validation
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